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Brain, pores and skin, eye, liver and coronary heart samples were subsequently excised and frozen in liquid N2 just before storing the samples at −eighty°C until finally later Investigation of lipid composition. Thus, a few pooled samples of 10 person organ/tissue samples (ten fish from Each individual tank, 30 fish in complete) were analysed for each diet plan team.
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The relative expression level of a marker protein (NeoR) under Smp1P was A great deal bigger than that under the ubiquitin promoter Utilized in a previous study35,39 (Supplementary Fig. S6b, c). Smp1P can be utilized for gene expression in not simply Parietichytrium sp. but will also other species of thraustochytrids, and so will support in the development of really efficient transformation systems for an array of thraustochytrids.
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